Recombinant interleukin 6
Inducer Abbreviation: rIL-6
Model Subtype: Biological
Model Species:Mus musculus
Model Subtype: Biological
Model Species:Mus musculus
Statistics Box:
No of Reports: 4
No of Models: 6
No of Reports: 4
No of Models: 6
Model Summary
A model to experimentally show that prenatal immune activation is an environmental risk factor for adult ventricular enlargement, works similar to poly(I:C) treament of mother at E12.5.
Human Clinical Evidence et al.
Strong epidemiological evidence suggests that maternal infection during prenatal life may contribute to an increased risk of schizophrenia or autism in offspring
Exposure
Prenatal exposure from mother
Mode of Action
Interleukin 6 is shown to be very important in the induction of ASD related phenotype in offspring exposed to maternal immune activation, via poly(I:C) treatment of mother
Usage
Immunostimulant
References
Type
Title
Author, Year
Primary
Maternal immune activation alters fetal brain development through interleukin-6.
Additional
The maternal interleukin-17a pathway in mice promotes autism-like phenotypes in offspring.
Additional
Effects of maternal immune activation on gene expression patterns in the fetal brain.
Additional
Melanotan-II reverses autistic features in a maternal immune activation mouse model of autism.
M_RIL6_1_IN_B
Construct Definition:
Administration of carrier-free mouse recombinant interleukin-6 (rIL-6) on E12.5.
Administration Dose: 5 microg
Administration Route: Intraperitoneal (i.p.)
Administration Age:
Strain of Origin: C57BL/6J
Model Source: Jackson Laboratory
Administration Dose: 5 microg
Administration Route: Intraperitoneal (i.p.)
Administration Age:
Strain of Origin: C57BL/6J
Model Source: Jackson Laboratory
M_RIL6_2_IN_B
Construct Definition:
Administration of carrier-free mouse recombinant interleukin-6 (rIL-6) on E12.5, the authors note that C57BL/6 mice used were of a stable immunological phenotype as they used these mice to study cytokine levels, therefore these are probably a substrain sold by Taconic.
Administration Dose: 10 microg
Administration Route: Intraperitoneal (i.p.)
Administration Age: E12.5
Strain of Origin: C57BL/6J
Model Source: Taconic, USA
Administration Dose: 10 microg
Administration Route: Intraperitoneal (i.p.)
Administration Age: E12.5
Strain of Origin: C57BL/6J
Model Source: Taconic, USA
M_RIL6_3_IN_B
Construct Definition:
Intraventricular injection, into the third ventricle of the fetal brain, of mouse recombinant, carrier-free IL-6 (R&D), 0.6 L of 10ng/L.
Administration Dose: 6 ng
Administration Route: Intraventricular
Administration Age: E14.5
Strain of Origin: C57BL/6J
Model Source: Taconic, USA
Administration Dose: 6 ng
Administration Route: Intraventricular
Administration Age: E14.5
Strain of Origin: C57BL/6J
Model Source: Taconic, USA
M_RIL6_4_IN_B
Construct Definition:
Pregnant C57BL/6J females were administered i.p. injections of 30 microgram/kg recombinant interleukin-6 (rIL-6) daily between E12.5 and E16.5.
Administration Dose: 30 microg/kg
Administration Route: Intraperitoneal (i.p.)
Administration Age: E12.5-E16.5
Strain of Origin: C57BL/6J
Model Source: Charles River
Administration Dose: 30 microg/kg
Administration Route: Intraperitoneal (i.p.)
Administration Age: E12.5-E16.5
Strain of Origin: C57BL/6J
Model Source: Charles River
M_RIL6_1_IN_B
Category
Entity
Quantity
Experimental Paradigm
Age at Testing
Sensorimotor gating
1
Decreased
Description:
Lower prepulse inhibition, compared to offspring from saline-injected dams
Exp Paradigm: Prepulse inhibition
Exp Paradigm: Prepulse inhibition
Prepulse inhibition
Adult
Tissue weight
2
Decreased
Description:
Decreased placental weight
Exp Paradigm: Placental weight measurements
Exp Paradigm: Placental weight measurements
Measurement of placental weight
Unreported
Cued or contextual fear conditioning: latent inhibition
1
Decreased
Description:
Lack of significant latent inhibition (increased freezing response), compared to offspring from saline-injected dams
Exp Paradigm: fear conditioning test: latent inhibition
Exp Paradigm: fear conditioning test: latent inhibition
Fear conditioning test
Adult
Gene expression
2
Abnormal
Description:
Abnormal transcriptome profile indicated by 195 genes differentially expressed in embryonic brain 3 h after injection
Exp Paradigm: Gene Expression
Exp Paradigm: Gene Expression
Gene expression microarray
E12.5
Gene expression
2
Increased
Description:
Increased gene expression of crystallin gene family: cryaa, cryba1, cryba2, cryba4, crybb1, crybb3, cryga and crygb/crygc in embryonic brain 3 h after injection
Exp Paradigm: Gene Expression
Exp Paradigm: Gene Expression
Gene expression microarray
E12.5
Not Reported:
Circadian sleep/wake cycle ,
Communications ,
Developmental profile ,
Emotion ,
Immune response ,
Learning & memory ,
Maternal behavior ,
Molecular profile ,
Motor phenotype ,
Neuroanatomy / ultrastructure / cytoarchitecture ,
Neurophysiology ,
Physiological parameters ,
Repetitive behavior ,
Seizure ,
Sensory ,
Social behavior ,
M_RIL6_2_IN_B
Category
Entity
Quantity
Experimental Paradigm
Age at Testing
Cortical lamination
1
Decreased
Description:
Cortical cytoarchitecture is disorganized, with 'intrusions' and 'protrusions', in offspring of mothers treated with rIL-6 at E12.5(similar to MIA via poly(I:C)), detected using markers: SATB2 (a marker for postmitotic neurons), TBR1(marker for deep cortical layers) and CTIP2 show disorganized expression
Immunohistochemistry
E18.5
Ultrasonic vocalization: isolation induced
1
Increased
Description:
rIL-6 treatment of mothers at E12.5 results in increased USV calls by pups, similar to poly(I:C) treatment for maternal immune activation
Exp Paradigm: Monitoring ultrasonic vocalizations: manual count
Exp Paradigm: Monitoring ultrasonic vocalizations: manual count
Cytokine levels: pro-inflammatory
1
Increased
Description:
rIL-6 treatment of mothers at E12.5 results in increased levels of IL-17a (pro-inflammatory cytokine) by E14.5 in the sera of mothers, similar to poly(I:C) treatment for maternal immune activation
Elisa
E14.5
Not Reported:
Circadian sleep/wake cycle ,
Developmental profile ,
Emotion ,
Learning & memory ,
Maternal behavior ,
Molecular profile ,
Motor phenotype ,
Neurophysiology ,
Physiological parameters ,
Repetitive behavior ,
Seizure ,
Sensory ,
Social behavior ,
M_RIL6_3_IN_B
Category
Entity
Quantity
Experimental Paradigm
Age at Testing
Not Reported:
Circadian sleep/wake cycle ,
Developmental profile ,
Emotion ,
Immune response ,
Learning & memory ,
Maternal behavior ,
Molecular profile ,
Motor phenotype ,
Neurophysiology ,
Physiological parameters ,
Repetitive behavior ,
Seizure ,
Sensory ,
Social behavior ,
M_RIL6_4_IN_B
Category
Entity
Quantity
Experimental Paradigm
Age at Testing
Neuroreceptor levels: oxt
1
Increased
Description:
MIA exposed adult male mice show increase in oxytocin receptor in the cingulate cortex region 1 including the anterior cingulate cortex compared with controls but no change of oxytocin receptor density was seen in the prelimbic cortex, anterior olfactory nucleus, nucleus accumbens, lateral septal nucleus, bed nucleus of the stria terminalis, ventral reuniens, paraventricular, and ventromedial hypothalamic nucleus.
Exp Paradigm: Anterior cingulate cortex, prelimbic cortex, anterior olfactory nucleus, nucleus accumbens, lateral septal nucleus, bed nucleus of the stria terminalis, ventral reuniens, paraventricular, and ventromedial hypothalamic nucleus
Exp Paradigm: Anterior cingulate cortex, prelimbic cortex, anterior olfactory nucleus, nucleus accumbens, lateral septal nucleus, bed nucleus of the stria terminalis, ventral reuniens, paraventricular, and ventromedial hypothalamic nucleus
Autoradiographic analysis
Adult
Self grooming: perseveration
1
Increased
Description:
MIA exposed adult male mice show increased grooming when placed in a beaker compared with controls.
Grooming behavior assessments
Adult
Social memory
1
Decreased
Description:
MIA exposed adult male mice show decrease in social novelty index compared with controls.
Three-chamber social approach test
Adult
Social approach
1
Decreased
Description:
MIA exposed adult male mice show decrease in sociability index compared with controls.
Three-chamber social approach test
Adult
Ultrasonic vocalization: interaction induced: opposite sex stimulus
1
Decreased
Description:
MIA exposed adult male mice show decrease in ultrasonic vocalization evoked by the presence of a C57 estrus female mouse compared with controls.
Monitoring ultrasonic vocalizations
Adult
Not Reported:
Circadian sleep/wake cycle ,
Developmental profile ,
Emotion ,
Immune response ,
Learning & memory ,
Maternal behavior ,
Molecular profile ,
Motor phenotype ,
Neurophysiology ,
Physiological parameters ,
Seizure ,
Sensory ,